Digital PCR is a new absolute quantitative PCR technique that mainly involves limited dilution of PCR reactants, followed by PCR amplification in different reaction chambers, and finally determining the initial copy number or concentration of target molecules based on the Poisson distribution principle and the number and proportion of positive droplets.

MicroDrop ™ Digital PCR can be widely applied in fields such as scientific research, medical care, and entry-exit inspection and quarantine.

Technical features:

Absolute quantification, independent of Ct values, no need for standard curves

Ultra high sensitivity, suitable for detecting rare sequences and rare mutations

Accurate quantitative results and excellent repeatability

Suitable for complex sample detection, not easily affected by PCR inhibitors

Core advantages:

High sensitivity——The sensitivity of digital PCR is directly proportional to the number of fractions in the same volume reaction system.

Resolution - as low as 0.1 times the difference in gene expression

Absolute Quantification - Calculate the absolute copy number of the detection sample based on the average copy number and Poisson distribution contained in each droplet. At the same time, there is no need to rely on standard curves.

MicroDrop ™ The microdrop-100A digital PCR system consists of a sample preparation instrument, a gene amplification instrument The MicroDrop-100B flow microdroplet detector and QuantDrop analysis software consist of four parts.

Detection principle:

MicroDrop ™ Workflow of droplet based digital PCR system:

Product Features of MicroDrop-100 System：

1、 Wide adaptability and high flexibility

1. Theoretically, it can cover qPCR (fluorescence quantitative PCR)

2. Sample diversity, sample throughput up to 96 samples, supporting flexible settings

3. An open platform that supports users to develop their own reagents and products.

2、 High sensitivity and accuracy

1. Absolute quantification - no need to rely on standard curves

2. Using droplet technology with up to 100000 droplets

3. The linear range reaches 6 orders of magnitude, and the sensitivity is as high as one ten thousandth

3、 Can analyze complex mixtures

1. Accuracy is not entirely dependent on amplification efficiency

2. Dual channel fluorescence detection, supporting EvaGreen dye and probe methods

3. Support multiple digital PCR

4、 Easy to operate and convenient to use

1. Fully enclosed anti pollution design, one click automated operation.

2. Optional all Chinese analysis software

Instrument features:

MicroDrop-100A Sample Preparation Instrument

1. A single chip can process 8 samples at a time;

2. Generate 100000 microdroplets in a 20 microliter system;

3. Single droplet generation only takes 2 minutes;

4. One click automated operation.

MD Detector Droplet Detector

1. Fully enclosed system, automated process operation;

2. FAM and VIC dual channel fluorescence detection;

3. The detection sensitivity is as high as one ten thousandth;

4. The flux reaches 96 samples.

MicroDrop-100: QuantDrop data analysis software

Application of Digital PCR

Inspection and Quarantine Food Safety

Genetically modified food testing (national standard)

• Identification of pathogenic bacteria

• Animal derived testing and analysis

scientific research

• Monitoring of gene expression differences

• Verification of CRISPR-Cas9 gene editing results

• Methylation content identification

Single cell research: sequencing PCR

clinical

• Tumor liquid biopsy: early screening, medication, monitoring, recurrence

• Non invasive prenatal screening: low-cost, fast

• infectious diseases: HBV, HIV quantification